1. Coverslip

• We recommend using high-precision cover glasses with thickness no. 1.5 to prepare the samples.
  • Zeiss 474030-9010-000 Decckläser 1,5H hi 100002240928 QS; Thickness no 1 ½ , high-performance 18 mm x 18 mm, 0.170 +/- 0,005 mm
  • Marienfeld Superior: www.marienfeld-superior.com
    • 18×18 mm (Cat.No. 0107032).22×22 mm (Cat.No. 0107052).
    • round with 18 mm diameter (Cat.No. 0117580).
• Fix your cells according to your preferred protocol.
• Please make sure that the cover slip is centered on the glass slide, in order to fit in our samples holder.
• For glass-bottom petri dishes and multi-well plates see:
  • Willco (http://www.willcowells.com/)
  • Lab-tek (offered through Thermo)
  • MatTek (glass bottom dishes
  • ibidi µ-Dish 35 mm, high Glass Bottom (Cat.No:81158)

2. Mounting Media

• Suggested Hardening media:
  • ProLong™ Glass Antifade Mount P36980 RI 1.52
  • ProLong™ Gold Antifade Mount   P36930 RI 1.46
• As it is a hardening media, you may also notice it can somewhat flatten samples.
• Do not use mounting media that include DAPI.
• Concerning the Refractive index (RI) of the mounting medium, prepare the slides at least 2 days before imaging. Allowing the sample to have sufficient time for curing mounting media and sealant. (2–8°C)
• The following Aqueous(Non-Hardening) Mounting liquid media also works, despite having a lower refractive index. SlowFadeGold /Diamond RI of 1,42. VectaShield H-1000. RI of 1,44

3. Fluorescent Labels for SR-SIM

All common types of organic dyes usually conjugated to antibodies or fluorescent proteins are suitable to be used for SR-SIM. For multicolor samples the fluorophores should be selected for minimal spectral overlap to avoid crosstalk.

• 405nm (50mW),
• 488nm (100mW),
• 561nm (100mW),
• 642nm (150mW)

Note: Cytosolic or other non-specific fluorescent protein expression (eg GFP) will result in staining of extended areas. Since well-defined structures are missing to interfere with the grid pattern, modulation contrast will be low and the final image will lack high resolution information.

＊This machine adopts the certification system＊

Can apply for the certification exam only after taking the principle and instrument introduction course, having confocal computer experience, and being taught by the commissioner on the actual machine operation, After passing the certification exam, those who have to operate more than 5 times (inclusive) during the daytime can have appointment and access control authority. Each person can make a maximum of 4 appointments within a two-week period, with a maximum of 4 hours per session.

• Reservation rules:
  • Please fill in the first time useSample Information Questionnaire, to be confirmed by the commissioner.
  • Please go to the instrument center before use"Instrument Reservation System"To make an appointment, the person who makes the appointment must be the actual user (it is not allowed to use other people's certificates to enter and exit).
  • The instrument must be cleaned and shut down after use, and the registration book must be filled out in detail. The start time is the appointment time, and the end time is based on the actual end-of-use time.
  • Do not delay boarding time or occupy other people's time.
  • Please confirm before 16:30 on the previous working day at the latest. Late changes will be regarded as violations and will not be accepted.
  • It is not possible to cancel the reservation on the same day. If the reservation time is less than half an hour, it will be regarded as a waiver.

If there is a need for long-term live cell shooting, please call the instrument manager to assist in making an appointment

• If you have the following qualifications, the fees will be compared with [our school teachers and students]:
  (1) Branch schools (2) Co-employed teachers (3) Affiliated hospitals (4) There are plans to cooperate with the school and the plans are under the control of the school
• If you have the following qualifications, the fee will be compared with the [Education Cooperation Unit]:
  (1) Part-time teachers (2) Teaching hospitals (Veterans General Hospital, Taipei City United Hospital, Yadong Hospital, etc.) (3) National Taiwan University System (4) Industry-University Operations Research Centers are stationed in manufacturers

The instrument is charged every half hour, bills will be billed at the end of every odd-numbered month, and within two weeks at the latest, please bring the payment slip to the cashier group of the administrative building to pay the bills and receive the receipt. If the number of overdue times reaches (including) two times, the laboratory will stop providing services.

1. This unit disables the pen drive. Due to the limited space on the hard disk of the computer, in order to facilitate the normal operation of the instrument, the management personnel will delete the data from time to time. The center is not responsible for data storage, please forgive me.
2. Without the consent of the administrator of the center,It is not allowed to add or remove any parts, change system settings and install software on the public instruments of the center without authorization. If any test is required for the experiment, the administrator's consent must be obtained first.Accompanied by the original engineer, the test will be carried out without harming the normal operation of the instrument. After the test is passed, it can be operated and used by the personnel approved by the administrator under the permission of the administrator of the center.
3. According to the judgment of the administrator, the violator will be warned for the first time, cancel the right of use for one month for the second time, and cancel the right of use for the third time. The right to use for one month, and those who violate the rules will be banned forever.

Instrument introduction:EN_product-info_Elyra-7

Instrument Operation Manual:Manual (20230314 version)

Certification Operation Application Form：Certification form

Principle and Instrument Introduction: The New Era of Super-resolution Microscopy, Elyra 7