Common Instruments | Optical Microscopes | Fluorescence Microscopes

Whole Slide Scanning System
Zeiss Axioscan 7

  • High-performance Slide Scanner for Fluorescence and Bright Field
  • Instrument Location: Room 438, 4th Floor, Biomedical Engineering Building
    ext 65980
  • Person in charge: Yangming CampusImage Core Facility Specialist
  • Qualification: Certification
    Only those who have passed the certification exam after teaching and have operated at least 5 times (inclusive) during the daytime period will have the right to make an appointment.
  • Charges:(tentative promotional price)300 yuan per hour for on-campus use, please refer to the charging standard for off-campus use
  • Reservation >>> Instrument Reservation System
microscope system
ZEISS Axioscan 7 Research Grade Fluorescent Slide Scanner 12-slice
objective lens
20X/0.5, 20X/0.8
fluorescent light source
Colibri 7 seven-channel LED light source: 385nm(UV), 430nm(V), 475nm(B), 555nm(G), 590nm(Y), 630nm(R), 735nm(Fr)
Ready to use

observation mode
Observation Methods
►Transmitted light: bright field (Bright Field); similar phase difference (PH) or similar differential interference contrast (DIC) relief image
►Fluorescence: five sets of fluorescent filters
Observation MethodExcitationEmission
DAPIUV (385nm)EM 425/30
GFPBlue (475nm) EM 514/30
DsRed/mCherryGreen (555nm) / Yellow (590nm) EM 592/25
Cy5Red (630nm)EM 681/45
Cy7Far Red (735nm) EM 788/38
camera equipment
This machine is a dual image system, which can shoot color images or black and white grayscale images
► Color CMOS: Axiocam 705 color, 5 million pixels
► Grayscale CMOS: Axiocam 712 mono, 12 million pixels
Slide size
slide holder
Mounting Frames
Accommodates standard slides (26 x 76mm), wide slides (52 x 76 mm)
Comes with three standard slide holders and one wide slide holder.
Three slide holders can be placed at one time, and can accommodate up to 12 standard slides, each of which can be set with individual shooting conditions and settings.

Apps and Features
Automatic scan area detection
auto focus
Batch scanning of transmitted light and fluorescent slides
automatic jigsaw puzzle
Z-stack and Extended Depth of Field extended depth of field image
video software
►ZEISS ZEN Blue: View scan images and download themFreeware ZEN Blue
►Offline computer workstation is available for image processing and analysis
►ZEN Module Intellesis + Image Analysis: Using machine learning methods (pixel classification and neural network) for image segmentation, as an alternative to traditional segmentation that is difficult to segment images
  • HE staining, silver staining Bright field sample observation
  • Fluorescent immunostaining samples
  • Support 4D image capture (XYZ λ)
  • Automatic scan detection/auto focus/auto mosaic
  • Batch scanning: up to 12 standard slides can be placed at one time, and different shooting parameters can be set for each slide
  • Z-stack motorized Z-axis scanning and Extended Depth of Field extended depth of field overlay processing
  • Simple manual measurement, or semi-automatic measurement after image segmentation with machine learning

*This machine adopts the certification system*

mustTeach first and pass certification exam, and operate 5 times (inclusive) or more by themselves during the daytime period to have the reservation authority. Each person can make a maximum of 4 appointments within a two-week period, with a maximum of 4 hours per session.

  1. Reservation rules:
    • Please go to the "Instrument Reservation System" of the Instrument Center to make an appointment before use.
    • After the instrument is used, it must be cleaned and turned off, and the registration book must be filled in in detail. The start time is the reservation time, and the end time is subject to the actual end of use time. Do not delay the time to get on the computer or occupy other people's time.
    • Please confirm before 16:30 on the previous working day at the latest. Late changes will be regarded as violations and will not be accepted.
    • It is not possible to cancel the reservation on the same day. If the reservation time is less than half an hour, it will be regarded as a waiver.
    • Please carefully evaluate the time required for scanning and reserve enough time for shooting so as not to affect the next user.
  2. Sample regulations:
    1. sample size(Be sure to ask the administrator if it is in line with the first use)
      A. Standard slide 76mm*26mm
      B. Wide slide 76mm*52mm
    2. It is only suitable for fixed slide samples, and the slide must be completely sealed and the slide must be cleaned before it can be used on the machine.
    3. from slide holderAlways use a loading aid when placing or removing slides.
    4. If you have other needs or special needs, please ask the administrator of the instrument center to provide relevant consulting services.
 Monday to Friday
all day
Restricted to certified operators for registration and use
Fluorescence MicroscopesWhole Slide Scanning SystemUprightinverted
For living cells
Mounted on Conjugate Focus Microscope
For living cells
model(Z) Axioscan 7(L) DM6000B
(O) BX61
(O) BX63
(O) IX83(Z) LSM880
(Z) LSM900
Hours per time (hours)4444
Hourly rate (NTD)On-campus teachers and students3000100300
Educational cooperation45050150450
other units600100200600
Remark1. Label Abbreviations: (L) Leica, (O) Olympus, (Z) Zeiss
3. Number of appointments: four appointments per person per instrument within two weeks
4. In addition to the education and training of the imaging core facilities organized by the center, it is recommended that you prepare your own samples (experimental data can be taken away) for reservations for teaching and certification exams, and the charges will be compared with the general use of the computer.
5. Charges are calculated on a half-hour basis.
2. These two units must be certified by the conjugation focus microscope before they can be used.
  • If you have the following qualifications, the fees will be compared with [our school teachers and students]:
    (1) Branch schools (2) Co-employed teachers (3) Affiliated hospitals (4) There are plans to cooperate with the school and the plans are under the control of the school
  • If you have the following qualifications, the fee will be compared with the [Education Cooperation Unit]:
    (1) Part-time teachers (2) Teaching hospitals (Veterans General Hospital, Taipei City United Hospital, Yadong Hospital, etc.) (3) National Taiwan University System (4) Industry-University Operations Research Centers are stationed in manufacturers

The instrument is charged every half hour, bills will be billed at the end of every odd-numbered month, and within two weeks at the latest, please bring the payment slip to the cashier group of the administrative building to pay the bills and receive the receipt. If the number of overdue times reaches (including) two times, the laboratory will stop providing services.

  1. This unit disables the pen drive. Due to the limited hard disk space of the computer, in order to facilitate the normal operation of the instrument,The management personnel will delete the data from time to time, and the center is not responsible for data storage, sorry.
  2. Without the consent of the administrator of the center,It is not allowed to add or remove any parts, change system settings and install software on the public instruments of the center without authorization. If any test is required for the experiment, the administrator's consent must be obtained first.Accompanied by the original engineer, the test will be carried out without harming the normal operation of the instrument. After the test is passed, it can be operated and used by the personnel approved by the administrator under the permission of the administrator of the center.
  3. According to the judgment of the administrator, the violator will be warned for the first time, cancel the right of use for one month for the second time, and cancel the right of use for the third time. The right to use for one month, and those who violate the rules will be banned forever.

Contact Imaging Core

Ms. Pei-Jun Chen
Extension 65980 / Instrumentation Resource Center, 4th Floor, Biomedical Engineering Building

Ms. Yung-yu Lu
Extension 66185 / Room 639, 6th Floor, Library, Information & Research Building

This article was last updated on March 6, 2023

Scroll to Top