Common Instruments | PCR Class

CFX Opus Real-Time PCR Systems
Real-time fluorescent quantitative PCR detection system

  • Instrument location
    • Room B02, Shou Yan Building (P machine)
  • Eligibility: Reservation required
    Free on-campus, please contact center staff when off-campus
  • Reservation >>> Instrument Reservation System


  1. Dynamic temperature gradient function: Gradient temperature control of "8 sections (A - H zones)" can be carried out at the same time. It can efficiently and quickly optimize the experimental reaction and find the ideal experimental conditions.
  2. Patented optical design – users can use the spectrum within the specified range to perform "single channel one by one excitation" and detect the fluorescent signal in each reaction well.
  3. Not only has higher sensitivity (1 copy of target sequence inhuman genome DNA), but also avoids fluorescent cross-interference between reactions, and can eliminate the influence of optical path difference at the same time (no need to use additional fluorescent ROX correction).
  4. Software analysis applications include
    • Absolute quantization
    • Relative quantization
    • Experimental results and plate analysis (Multiple plates combination)
    • Multiple reference genes
    • Efficiency estimation
    • Genotyping (Allelic discrimination, SNP)
    • End point analysis function
  5. Automated analysis of software statistics: through the quick setting of p-value, it is possible to easily complete "t-test" and "One Way ANOVA" analysis with one click.
  1. A reservation is required to use this machine in advance. (please refer to reservation policy)
  2. Before boarding the aircraft, you should fill in all the information on the registration book, and take back the samples as soon as possible after boarding, otherwise, please be responsible for any damage to the samples.
  3. Users are not allowed to perform the following operations on the computers of the center:
    • A. Change programs or parameters, or other people's files.
    • B. Externally connect any peripheral devices.
    • C. Copy the program to the central computer for execution (the programs in the central computer are all original and have copyright).
    • Violators will stop using it forever, and if any damage is caused, they will be punished according to the school rules.
  4. If you have any questions when you get on the machine, please ask the instrument center technicians.
  5. Restricted0.1ml PCR 96 well plate or tube (only Low Profile tube or plate can be used) If you use other specifications to cause damage to the machine, you will be responsible for repairs!
  1. The person making the reservation must be the real user and cannot be used as a substitute.
  2. The earliest appointment is two weeks in advance. (Example: Today's Tuesday can only be reserved until next Monday at the earliest)
  3. Each person can book a maximum of two time slots at a time, and there must be an interval, and each time slot cannot exceed 3 hours.
  4. If you do not use it for more than 30 minutes within the reservation period, it will be regarded as giving up the use, and others can use it at any time.
  5. The access or return time of accessory accessories shall not affect the use of others.
  6. Please take your personal belongings away and do not leave samples.

※ Cancellation of the reservation must be cancelled as soon as possible, so as not to damage the rights and interests of others! If there are frequent appointments without prior cancellation, the center will strictly limit the use of those who are suspected of habitual occupancy after investigation.

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Contact our staff


Miss Chen Wenling
ext 62382

Ms. Yu-Chieh Chien
ext 62382/65660

This article was last updated on May 12, 2023

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